Non Animal Testing, Alternative Test Methods, In Vitro Toxicology, IIVS | Assays Cytotoxicity
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Archive

Webinar: How GLPs Enhance the Quality of Regulated and Non-Regulated Toxicology

October 18, 2016
This one-hour webinar, led by IIVS Director of Quality and Compliance, introduces some of the concepts of Good Laboratory Practices (GLPs) designed to promote study and data integrity within an in vitro toxicology framework. Applying these concepts within your own laboratory should aid in production of robust, repeatable studies. View Slides...

Reconstructed Human Epidermis (RHE) Models

August 24, 2016
The 3-D Phototoxicity assay uses 3-D reconstructed human epidermal (RHE) tissue constructs to evaluate the dermal phototoxicity potential of a test material. Toxicity is determined by measuring cytotoxicity [based on the relative conversion of MTT (3-[4,5 - dimethylthiazol-2-yl] - 2,5 - diphenyltetrazolium bromide)] in cultures treated with the test material in the presence or absence of UVA light. An advantage of using 3-D tissues to evaluate phototoxicity potential is that, unlike the 96-well phototoxicity assay, test materials are applied topically, so solids,...

3T3 Neutral Red Uptake (OECD 432)

August 24, 2016
The 3T3 Neutral Red Uptake (NRU) Phototoxicity assay is a 96-well cytotoxicity-based assay that utilizes normal BALB/c 3T3 mouse fibroblasts to measure the concentration-dependent reduction in neutral red uptake by the cells after exposure to a test material either in the presence or absence of UVA light. Duplicate 96-well monolayers of 3T3 fibroblasts are exposed to serial dilutions of a test material. One of the plates is exposed to 5 J/cm2 UVA while the other plate is kept in the...

Medical Devices

August 24, 2016
IIVS offers in vitro cytotoxicity assay using Modified Eagles Medium (MEM) elution and agar overlay for medical devices.  These in vitro biological reactivity assays, MEM Elution and Agar Diffusion, meet the United States Pharmacopoeia (USP) and International Organization for Standardization (ISO) 10993 requirements. Clients include medical devices, consumer products, and cosmetic companies with products defined as medical devices....

NHEK & 3T3

August 22, 2016
The 3T3 Neutral Red Uptake (NRU) Cytotoxicity Assay using a Neutral Red Uptake (NRU) viability endpoint is a 96-well cytotoxicity assay to assess the toxicity potential of a test material. The assay utilizes Balb/c 3T3 mouse fibroblasts to assess the systemic toxicity potential of a test material and may be used to predict in vivo rodent LD50 starting doses for acute oral systemic toxicity. Cytotoxicity caused by the test article is measured by a concentration-dependent reduction in neutral red dye uptake by the...

AGAR Overlay

August 22, 2016
The agar overlay assay or agar diffusion test is an in vitro cytotoxicity assay designed to show the presence of toxic material eluted from a test sample as it affects L929 cells cultured in the presence of the test article. Test articles are placed onto the agar surface overlaid onto a L929 cell monolayer preventing direct damage while allowing diffusion of leachable chemicals from the sample. After treatment, the cells are exposed to neutral red dye to differentiate between the viable...

Neutral Red Uptake

August 21, 2016
The Normal Human Epidermal Keratinocytes (NHEKs) Cytotoxicity Assay using a Neutral Red Uptake (NRU) viability endpoint is a 96-well cytotoxicity assay to assess the toxicity potential of a test material. The assay utilizes primary NHEK cells and may be used to predict either human lethal serum concentrations or in vivo rodent LD50 starting doses for acute oral systemic toxicity. Cytotoxicity caused by the test article is measured by a concentration-dependent reduction in neutral red uptake (NRU) by the cells after exposure to a...

MEM Elution

August 21, 2016
The MEM elution assay or elution test is an in vitro cytotoxicity assay designed to show the presence of toxic material eluted from a test sample as it affects L929 cells cultured in the presence of the extract. Extracts of test articles are applied to L929 cells.  After treatment, the cells are exposed to neutral red dye to differentiate between the viable and stressed or lysed cells.  The degree of irritancy is determined microscopically in the percentage of cells deformed and...

Toxicity Screens

August 2, 2016
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