Non Animal Testing, Alternative Test Methods, In Vitro Toxicology, IIVS | Assays Skin Sensitization
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Archive

Modernization of EPA “6-Pack” of Acute Toxicity Tests Continues

July 26, 2018
The US Environmental Protection Agency (US EPA) continues to drive significant efforts in the United States to modernize the battery of acute toxicity tests classically known as the “6-pack.” Known for many years as an “all in vivo” testing strategy for products registered under the Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA), the 6-pack was revised in 2015 to include non-animal (in vitro/ex vivo) test methods for classification and labeling for Eye Irritation, both for commonly used household cleaning products...

Toxicity Testing: Read Between the Lines

April 13, 2017
Skin toxicity testing  was the focus of a full-day seminar held during In-Cosmetics 2017. Attendees learned about everything from skin absorption and metabolism, to cytotoxicity, phototoxicity and sensitization. IIVS Study Director, Dr. Tinashe Ruwona, presented on integrated testing strategies to evaluate the skin sensitization potential of cosmetics. However, the biggest take-away from the seminar seemed to rest on how well the results of a study are understood. Read the full article.  ...

IIVS Attends In-Cosmetics Global

April 4, 2017
IIVS President, Erin Hill, and Study Director, Tinashe Ruwona are attending the In-Cosmetics Global meeting, April 4-6, in London.  Dr. Ruwona, who leads our skin sensitization screening program, presented during the Skin Toxicology Workshop. His presentation,  "Integrated Testing Strategies for Assessing the Skin Sensitization Potential of Cosmetics",  Dr. Ruwona explores the various test methods and how best to combine them to ensure consumer safety.  Read more....

In Vitro Assessment of Skin Irritation Potential of Surfactant-based Formulations by Using a 3-D Skin Reconstructed Tissue Model and Cytokine Response

January 24, 2017
The personal care industry is focused on developing safe, more efficacious, and increasingly milder products, that are routinely undergoing preclinical and clinical testing before becoming available for consumer use on skin. In vitro systems based on skin reconstructed equivalents are now established for the preclinical assessment of product irritation potential and as alternative testing methods to the classic Draize rabbit skin irritation test. We have used the 3-D EpiDerm™ model system to evaluate tissue viability and primary cytokine interleukin-1α release...

Webinar: How GLPs Enhance the Quality of Regulated and Non-Regulated Toxicology

October 18, 2016
This one-hour webinar, led by IIVS Director of Quality and Compliance, introduces some of the concepts of Good Laboratory Practices (GLPs) designed to promote study and data integrity within an in vitro toxicology framework. Applying these concepts within your own laboratory should aid in production of robust, repeatable studies. View Slides...

LuSens (OECD 442D)

August 24, 2016
Similar to the KeratinoSens assay (OECD 442D), also offered by IIVS, LuSens addresses the cellular event of Keratinocyte activation, the second key event that triggers the induction of skin sensitization.  It is a luciferase-assay bioassay performed in a human keratinocyte cell line.  The approach is based on evaluating the upregulation of a gene involved in the induction of the sensitization response. IIVS participated in an inter-laboratory study led by BASF to assess the transferability, reproducibility, and accuracy of the method.  The...

Human Cell Line Activation Test (h-CLAT, OECD 442E)

August 24, 2016
The human Cell Line Activation Test (h-CLAT) is a cell-based assay that identifies skin sensitizers by examining changes in the expression of cell surface markers (CD54 and CD86) implicated in dendritic cell activation, the third key event of the skin sensitization AOP.  Following exposure of the THP-1 human monocyte cell line to the test substance, expression levels of CD54 and CD86 are quantified by flow cytometry and compared to controls. The method has been reviewed by the European Union Reference Laboratory...

KeratinoSens (OECD 442D)

August 24, 2016
The KeratinoSensTM assay is a cell-based reporter gene assay that models the second key event in the Adverse Outcome Pathway for Dermal Sensitization, namely keratinocyte activation.  This approach measures the induction of luciferase under the control of the antioxidant response element (ARE) derived from the human AKR1C2 gene, and can be used in a Weight of Evidence approach as an alternative to animal testing methods for the purpose of hazard identification. IIVS has participated in validation studies led by the assay developer...

Next Generation Tobacco Products

August 24, 2016
IIVS has a long-standing history of working with various industries to help establish or utilize in vitro models for the prediction of risk and the assessment of products under development. With the Food and Drug Administration extending its authority to all tobacco products (including e-cigarettes, cigars, and hookah) the tobacco industry is faced with the task of assessing product safety for product development and regulatory submission. Further, the manufacture of tobacco products may entail risk to workers that come in...

Cosmetics & Personal Care

August 24, 2016
European legislation and US research activities towards ‘21st century toxicology’ are setting the standards for animal-free toxicological assessments and the cosmetics industry has been the first to feel the effects. As per EU Regulation 1223/2009, animal testing of cosmetic ingredients and finished products is currently banned in the EU, as is the marketing of finished cosmetic products and the ingredients contained therein which were tested on animals for cosmetic safety assessment purposes. These bans hold irrespective of the availability of validated...