IIVS, Non Animal Testing, Alternative Test Methods, In Vitro Testing | Assays UV Induced Toxicity
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Webinar: How GLPs Enhance the Quality of Regulated and Non-Regulated Toxicology

October 18, 2016
This one-hour webinar, led by IIVS Director of Quality and Compliance, introduces some of the concepts of Good Laboratory Practices (GLPs) designed to promote study and data integrity within an in vitro toxicology framework. Applying these concepts within your own laboratory should aid in production of robust, repeatable studies. View Slides...

Tissue Models

August 24, 2016
The 3-D Phototoxicity assay uses 3-D reconstructed human epidermal (RHE) tissue constructs to evaluate the dermal phototoxicity potential of a test material. Toxicity is determined by measuring cytotoxicity [based on the relative conversion of MTT (3-[4,5 - dimethylthiazol-2-yl] - 2,5 - diphenyltetrazolium bromide)] in cultures treated with the test material in the presence or absence of UVA light. An advantage of using 3-D tissues to evaluate phototoxicity potential is that, unlike the 96-well phototoxicity assay, test materials are applied topically, so solids,...

3T3 Neutral Red Uptake (OECD 432)

August 24, 2016
The 3T3 Neutral Red Uptake (NRU) Phototoxicity assay is a 96-well cytotoxicity-based assay that utilizes normal BALB/c 3T3 mouse fibroblasts to measure the concentration-dependent reduction in neutral red uptake by the cells after exposure to a test material either in the presence or absence of UVA light. Duplicate 96-well monolayers of 3T3 fibroblasts are exposed to serial dilutions of a test material. One of the plates is exposed to 5 J/cm2 UVA while the other plate is kept in the...

UV Induced Toxicity

August 23, 2016
Test materials that absorb light in the UVB, UVA, and visible light range and are topically applied to or may be distributed to sun exposed areas of the skin should be considered for photosafety evaluation.  Test materials that are phototoxic can produce adverse effects in the presence of light after exposure. At IIVS, we perform two in vitro assays that are routinely used to evaluate phototoxicity hazard and risk. The 3T3 Neutral Red Uptake (NRU) Phototoxicity Assay (OECD Test Guideline 432) is...