IIVS | Assays Ocular
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Registration Open for 2018 Practical Methods Workshop

August 23, 2017
Registration is now open for the 2018 Practical Methods in In Vitro Toxicology Workshop, Jan,16-19, at IIVS in Gaithersburg, MD. During this four-day intensive workshop, you will gain the scientific information and expertise you need to strategically integrate non-animal methods within your testing program.  Register now....

Webinar: How GLPs Enhance the Quality of Regulated and Non-Regulated Toxicology

October 18, 2016
This one-hour webinar, led by IIVS Director of Quality and Compliance, introduces some of the concepts of Good Laboratory Practices (GLPs) designed to promote study and data integrity within an in vitro toxicology framework. Applying these concepts within your own laboratory should aid in production of robust, repeatable studies. View Slides...

Cytosensor Microphysiometer

August 24, 2016
The Cytosensor Microphysiometer assay is an in vitro cellular toxicity test used to evaluate ocular irritancy. A microphysiometer is used to detect and monitor the extracellular changes in pH in L929 (mouse fibroblast) cells after exposure to a test material. Changes in the pH are caused by variations in the metabolic rate, measured indirectly as a function of changes in extracellular acidification. Metabolism in living cells is tightly coupled to cellular ATP usage and extracellular release of acidic byproducts such as protons, lactic...

Ocular Irritection (OIA)

August 24, 2016
The Ocular Irritection Assay is an in chemico eye irritation assay that detects, ranks, and predicts the corneal irritation potential of a test material. The assay assesses changes to the reagent solution (containing proteins, glycoproteins, lipids), which mimics the denaturation and disruption that occurs in corneal proteins in vivo. The test article is applied to a membrane disc that controls the delivery to the reagent solution. The changes in the protein structure are measured by optical density readings in the reagent...

Trans-Epithelial Permeability/Fluorescein Leakage (OECD 460)

August 24, 2016
The Trans-Epithelial Permeability (TEP) Assay, is a cell-based assay used to evaluate the potential ocular irritancy of test chemicals by measuring the permeability of sodium fluorescein (or fluorescein leakage) through a confluent monolayer of Madin-Darby Canine Kidney (MDCK) cells. The MDCK cell line is used since it forms tight junctions in a confluent monolayer similar to those formed in the outermost corneal and conjunctival epithelial layers. The disruption of the tight junctions by chemicals is one of the early events typical...

CAMVA

August 24, 2016
The CAMVA is an in vitro assay that measures the presence and degree of vascular effect to the chorioallantoic membrane (CAM) of 10-day fertilized eggs following exposure to test article. After exposure, the CAM is examined visually for vascular hemorrhaging, capillary injection and/or ghost vessels at the test material application site. The concentration that elicits a positive response in 50% of the treated CAM specimens (RC50) indicates ocular irritation potential of a test material. Exposed CAM can also be evaluated for...

Short Time Exposure (STE, OECD 491)

August 24, 2016
The Short Time Exposure (STE) assay, developed by Kao Corporation (Japan), is an in vitro assay used to assess acute eye irritation potential as an alternative to the traditional in vivo Draize test. The test method evaluates the cytotoxicity induced by a series of test chemical dilutions in a monolayer of rabbit corneal fibroblasts (Statens Seruminstitut Rabbit Cornea – SIRC) after a single five-minute exposure. Two prediction models were initially developed for the STE assay – one categorizes the test material...

NociOcular Eye Sting

August 24, 2016
The NociOcular Assay predicts the eye stinging potential of materials, such as cosmetics, sunscreens, surfactants, and ophthalmic products. The assay relies on the expression of functional Transient Receptor Potential Vanilloid type 1 (TRPV1) channels in a clone of the human SH-SY5Y neuroblastoma cell line. The TRPV1 channel is a well characterized pain receptor that is present in tissues associated with the eye. This channel can be activated by chemical stimuli and is thought to be a general mediator of chemically...

Assessing Corneal Recovery

August 24, 2016
Corneal recovery is a key property of ocular irritation classification schemes used in the regulatory arena. However, no current validated non-animal ocular irritation test methods or prediction tools are able to predict corneal recovery in a manner consistent with the animal tests historically employed. Whereas some efforts have been made to demonstrate recovery with in vitro or ex vivo corneal models, the current state of these technologies do not support demonstration of recovery of the corneal stromal elements, which are...

Eye Irritation Test (EIT, OECD 492)

August 24, 2016
The Eye Irritation Test (EIT) is an OECD-approved in vitro non-animal test method for identifying chemicals and mixtures not requiring classification and labelling for eye irritation or serious eye damage. The test method utilizes an in vitro reconstructed human corneal epithelium (RhCE) model (EpiOcular™, MatTek Corp.), in an acute exposure assay to support international regulatory labeling requirements, according to the United Nations Globally Harmonized System of Classification and Labelling of Chemicals (UN GHS). The Eye Irritation Test can be used to...