Non Animal Testing, Alternative Test Methods, In Vitro Toxicology, IIVS | Assays Trans-Epithial Permeability
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Archive

Webinar: How GLPs Enhance the Quality of Regulated and Non-Regulated Toxicology

October 18, 2016
This one-hour webinar, led by IIVS Director of Quality and Compliance, introduces some of the concepts of Good Laboratory Practices (GLPs) designed to promote study and data integrity within an in vitro toxicology framework. Applying these concepts within your own laboratory should aid in production of robust, repeatable studies. View Slides...

Cytosensor Microphysiometer

August 24, 2016
The Cytosensor Microphysiometer assay is an in vitro cellular toxicity test used to evaluate ocular irritancy. A microphysiometer is used to detect and monitor the extracellular changes in pH in L929 (mouse fibroblast) cells after exposure to a test material. Changes in the pH are caused by variations in the metabolic rate, measured indirectly as a function of changes in extracellular acidification. Metabolism in living cells is tightly coupled to cellular ATP usage and extracellular release of acidic byproducts such as protons, lactic...

Trans-Epithelial Permeability/Fluorescein Leakage (OECD 460)

August 24, 2016
The Trans-Epithelial Permeability (TEP) Assay, is a cell-based assay used to evaluate the potential ocular irritancy of test chemicals by measuring the permeability of sodium fluorescein (or fluorescein leakage) through a confluent monolayer of Madin-Darby Canine Kidney (MDCK) cells. The MDCK cell line is used since it forms tight junctions in a confluent monolayer similar to those formed in the outermost corneal and conjunctival epithelial layers. The disruption of the tight junctions by chemicals is one of the early events typical...

Bovine Corneal Opacity and Permeability (OECD 437) & Histology

August 24, 2016
The BCOP (Bovine Corneal Opacity and Permeability) assay is an in vitro eye irritation test method developed by Gautheron et al. (1992), which uses living bovine corneal tissue, obtained as a by-product from abattoirs, to evaluate the potential ocular irritancy of a test article. Types of injury caused by exposure to the test article are quantitatively measured by changes in opacity and permeability to fluorescein. The BCOP assay allows for the investigation of the mechanism of the damage caused. Corneal opacity...