Non Animal Testing, Alternative Test Methods, In Vitro Toxicology, IIVS | Assays Sensitization
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Archive

IIVS Collaborates with BASF and Givaudan to Validate a Non-animal Test for Regulatory Submissions

August 28, 2018
GAITHERSBURG, MD –August 28– The Institute for In Vitro Sciences (IIVS), with an established track record in the validation of alternatives to animal testing, is teaming up with BASF and Givaudan to validate an improved skin sensitization reactivity method to address the needs of toxicologists and regulators. Scientists at BASF and Givaudan have developed the Kinetic Direct Peptide Reactivity Assay (Kinetic DPRA), a non-animal test to predict allergic reactions in the skin known as sensitization. Three other non-animal sensitizer tests have...

Registration Now Open for Practical Methods 2019

August 27, 2018
IIVS is excited to announce that registration is now open for our 2019 Practical Methods for In Vitro Toxicology Workshop. Space is limited so please book as soon as possible to guarantee your spot. ...

Modernization of EPA “6-Pack” of Acute Toxicity Tests Continues

July 27, 2018
The US Environmental Protection Agency (US EPA) continues to drive significant efforts in the United States to modernize the battery of acute toxicity tests classically known as the “6-pack.” Learn More....

The Assessment of Phototoxicity Using the 3T3 Neutral Red Uptake (NRU) Phototoxicity Assay and a Modified Photo-Direct Peptide Reactivity Assay (DPRA)

March 28, 2018
Alternative methods, including the validated 3T3 Neutral Red Uptake (NRU) Phototoxicity assay (OECD TG 432) may be used as a pre-clinical test to address phototoxicity. Currently, there are no validated alternative test methods to identify photoallergens; however, there are several validated alternative test methods to address skin sensitization, including the Direct Peptide Reactivity Assay (DPRA) (OECD 442C). To address photoallergy, we utilized the 3T3 Phototoxicity assay in combination with a modified photo-DPRA assay to determine if these assays were able...

IIVS and BASF Collaborate to Distribute Test Method to Replace Animal Testing in China

January 9, 2018
GAITHERSBURG, MD – January 9, 2018 – The Institute for In Vitro Sciences, Inc. (IIVS) today announced a collaboration with BASF to import a non-animal, cell based, safety test into China. The LuSens test, developed by BASF, is an in vitro test to detect the sensitization potential of chemicals to human skin. The test was developed to be license free and shared with the scientific community at-large. It has recently been incorporated into the Organization for Economic Co-operation and Development...

Webinar: How GLPs Enhance the Quality of Regulated and Non-Regulated Toxicology

October 18, 2016
This one-hour webinar, led by IIVS Director of Quality and Compliance, introduces some of the concepts of Good Laboratory Practices (GLPs) designed to promote study and data integrity within an in vitro toxicology framework. Applying these concepts within your own laboratory should aid in production of robust, repeatable studies. View Slides...

Integrated Testing Strategies for Skin Sensitization

August 24, 2016
Determination of skin sensitization potential is a critical endpoint in the safety assessment of raw materials, chemicals, mixtures and formulations.  Although the Guinea Pig Maximization Test (GPMT) and Local Lymph node assay (LLNA) have historically been used to address this adverse effect, in vitro assays have been developed and validated in order to replace these resource-intensive animal tests. Skin sensitization is the result of a series of biochemical "Key Events" (KEs) that involve covalent binding of (generally electrophilic) compounds to cellular...

LuSens (OECD 442D)

August 24, 2016
Similar to the KeratinoSens assay (OECD 442D), also offered by IIVS, LuSens addresses the cellular event of Keratinocyte activation, the second key event that triggers the induction of skin sensitization.  It is a luciferase-assay bioassay performed in a human keratinocyte cell line.  The approach is based on evaluating the upregulation of a gene involved in the induction of the sensitization response. IIVS participated in an inter-laboratory study led by BASF to assess the transferability, reproducibility, and accuracy of the method.  The...

Human Cell Line Activation Test (h-CLAT, OECD 442E)

August 24, 2016
The human Cell Line Activation Test (h-CLAT) is a cell-based assay that identifies skin sensitizers by examining changes in the expression of cell surface markers (CD54 and CD86) implicated in dendritic cell activation, the third key event of the skin sensitization AOP.  Following exposure of the THP-1 human monocyte cell line to the test substance, expression levels of CD54 and CD86 are quantified by flow cytometry and compared to controls. The method has been reviewed by the European Union Reference Laboratory...

Direct Peptide Reactivity Activation Assay (DPRA, OECD 442C)

August 24, 2016
The DPRA  (OECD 442C) is an in chemico assay that  models the first key event in the skin sensitization Adverse Outcome Pathway (AOP) - skin, protein reactivity. Compounds implicated in causing Allergic Contact Dermatitis (ACD) are generally electrophilic in nature. This assay identifies dermal sensitizers based on their reactivity with synthetic peptides containing the nucleophilic amino acid residues lysine and cysteine. Using LC/UV, conjugation of the test material with the peptides can be analyzed. Trained by the developers of this assay (Procter &...