IIVS | Assays Itegrated Testing Strategies
89
archive,tax-assay,term-itegrated-testing-strategies,term-89,ajax_fade,page_not_loaded,,qode_grid_1300,footer_responsive_adv,hide_top_bar_on_mobile_header,qode-content-sidebar-responsive,qode-child-theme-ver-,qode-theme-ver-11.0,qode-theme-bridge,wpb-js-composer js-comp-ver-4.11.2.1,vc_responsive

Archive

Webinar: How GLPs Enhance the Quality of Regulated and Non-Regulated Toxicology

October 18, 2016
This one-hour webinar, led by IIVS Director of Quality and Compliance, introduces some of the concepts of Good Laboratory Practices (GLPs) designed to promote study and data integrity within an in vitro toxicology framework. Applying these concepts within your own laboratory should aid in production of robust, repeatable studies. View Slides...

Integrated Testing Strategies

August 24, 2016
Determination of skin sensitization potential is a critical toxicological endpoint in the safety assessment of novel chemicals. Although the Guinea Pig Maximization Test (GPMT) and in vivo Local Lymph node assay (LLNA) have traditionally been used to assess skin sensitization, recent activity has focused on the development of novel alternative assays for the endpoint. As a culmination of these efforts, the Organization for Economic Cooperation and Development (OECD) published the following test guidelines for non-animal skin sensitization testing on 5 February...

Direct Peptide Reactivity Activation Assay (DPRA, OECD 442C)

August 24, 2016
The DPRA is an in chemico assay that identifies dermal sensitizers based on their reactivity with synthetic peptides containing either lysine or cysteine. The assay models the first key event, protein reactivity, in the skin sensitization AOP. Through collaboration with the method developers at Procter & Gamble, IIVS scientists received training, successfully demonstrated regulatory-required proficiency, and are offering the assay as a commercial service. IIVS is currently exploring the use of the OECD approved DPRA method (OECD 442C) in combination with other assays, including...

KeratinoSens (OECD 442D)

August 24, 2016
The KeratinoSensTM assay is a cell-based reporter gene assay which identifies skin sensitizers by measuring the induction of luciferase under the control of the antioxidant response element (ARE) derived from the human AKR1C2 gene. In the proposed Adverse Outcome Pathway (AOP) leading to skin sensitization, this method addresses the second key event, gene expression in keratinocytes associated with the antioxidant/electrophile response element (ARE)-dependent pathway. The IIVS laboratory participated in the validation studies led by Givaudan to assess the KeratinoSensTM assay which demonstrated...

Fragrance & Flavors

August 24, 2016
Exposure to fragrance chemicals is most commonly by direct skin contact. Allergic contact dermatitis (ACD) to fragrance ingredients is most often caused by cosmetic products, such as perfumes, deodorants, and aftershave products. Cosmetic fragrance ingredients should be assessed for exposure damage including skin sensitization (ACD), phototoxicity, skin irritation (irritant contact dermatitis), and respiratory irritation and sensitization. At IIVS, we have pre-clinical testing programs to address each of these endpoints to ensure the safety of fragrance ingredients.JTNDJTIxLS1CZWdpbiUyMENUQ1QlMjBTaWduLVVwJTIwRm9ybS0tJTNFJTBBJTNDJTIxLS0lMjBFRkQlMjAxLjAuMCUyMCU1QlRodSUyMEphbiUyMDEyJTIwMTAlM0EyMyUzQTU2JTIwRVNUJTIwMjAxNyU1RCUyMC0tJTNFJTBBJTNDJTIxLS0lMjAlM0NsaW5rJTIwcmVsJTNEJTI3c3R5bGVzaGVldCUyNyUyMHR5cGUlM0QlMjd0ZXh0JTJGY3NzJTI3JTIwaHJlZiUzRCUyN2h0dHBzJTNBJTJGJTJGc3RhdGljLmN0Y3RjZG4uY29tJTJGaCUyRmNvbnRhY3RzLWVtYmVkZGVkLXNpZ251cC1hc3NldHMlMkYxLjAuMiUyRmNzcyUyRnNpZ251cC1mb3JtLmNzcyUyNyUzRSUyMC0tJTNFJTBBJTNDZGl2JTIwY2xhc3MlM0QlMjJjdGN0LWVtYmVkLXNpZ251cCUyMiUyMHN0eWxlJTNEJTIyZm9udCUzQSUyMDE2cHglMjBIZWx2ZXRpY2ElMjBOZXVlJTJDJTIwQXJpYWwlMkMlMjBzYW5zLXNlcmlmJTNCJTIwZm9udCUzQSUyMDFyZW0lMjBIZWx2ZXRpY2ElMjBOZXVlJTJDJTIwQXJpYWwlMkMlMjBzYW5zLXNlcmlmJTNCJTIwbGluZS1oZWlnaHQlM0ElMjAxLjUlM0IlMjAtd2Via2l0LWZvbnQtc21vb3RoaW5nJTNBJTIwYW50aWFsaWFzZWQlM0IlMjIlM0UlMEElMjAlMjAlMjAlM0NkaXYlM0UlMEElMjAlMjAlMjAlMjAlMjAlMjAlMjAlM0NzcGFuJTIwaWQlM0QlMjJzdWNjZXNzX21lc3NhZ2UlMjIlMjBzdHlsZSUzRCUyMmRpc3BsYXklM0Fub25lJTNCJTIyJTNFJTBBJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTNDZGl2JTIwc3R5bGUlM0QlMjJ0ZXh0LWFsaWduJTNBY2VudGVyJTNCJTIyJTNFVGhhbmtzJTIwZm9yJTIwc2lnbmluZyUyMHVwJTIxJTNDJTJGZGl2JTNFJTBBJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTNDJTJGc3BhbiUzRSUwQSUyMCUyMCUyMCUyMCUyMCUyMCUyMCUzQ2Zvcm0lMjBkYXRhLWlkJTNEJTIyZW1iZWRkZWRfc2lnbnVwJTNBZm9ybSUyMiUyMGNsYXNzJTNEJTIyY3RjdC1jdXN0b20tZm9ybSUyMEZvcm0lMjIlMjBuYW1lJTNEJTIyZW1iZWRkZWRfc2lnbnVwJTIyJTIwbWV0aG9kJTNEJTIyUE9TVCUyMiUyMGFjdGlvbiUzRCUyMmh0dHBzJTNBJTJGJTJGdmlzaXRvcjIuY29uc3RhbnRjb250YWN0LmNvbSUyRmFwaSUyRnNpZ251cCUyMiUzRSUwQSUyMCUyMCUyMCUyMCUyMCUyMCUyMCUyMCUyMCUyMCUyMCUzQ2gzJTIwc3R5bGUlM0QlMjJtYXJnaW4lM0EwJTNCJTIyJTNFR2V0JTIwb3VyJTIwbmV3c2xldHRlciUzQyUyRmgzJTNFJTBBJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTNDcCUzRSUzQyUyRnAlM0UlMEElMjAlMjAlMjAlMjAlMjAlMjAlMjAlMjAlMjAlMjAlMjAlM0MlMjEtLSUyMFRoZSUyMGZvbGxvd2luZyUyMGNvZGUlMjBtdXN0JTIwYmUlMjBpbmNsdWRlZCUyMHRvJTIwZW5zdXJlJTIweW91ciUyMHNpZ24tdXAlMjBmb3JtJTIwd29ya3MlMjBwcm9wZXJseS4lMjAtLSUzRSUwQSUyMCUyMCUyMCUyMCUyMCUyMCUyMCUyMCUyMCUyMCUyMCUzQ2lucHV0JTIwZGF0YS1pZCUzRCUyMmNhJTNBaW5wdXQlMjIlMjB0eXBlJTNEJTIyaGlkZGVuJTIyJTIwbmFtZSUzRCUyMmNhJTIyJTIwdmFsdWUlM0QlMjI0OGY2ZWQyMi00ZDEwLTQyY2QtOGFkMi01NWM4NTQ0OTMxYzYlMjIlM0UlMEElMjAlMjAlMjAlMjAlMjAlMjAlMjAlMjAlMjAlMjAlMjAlM0NpbnB1dCUyMGRhdGEtaWQlM0QlMjJsaXN0JTNBaW5wdXQlMjIlMjB0eXBlJTNEJTIyaGlkZGVuJTIyJTIwbmFtZSUzRCUyMmxpc3QlMjIlMjB2YWx1ZSUzRCUyMjE0MjQ1NDkxNTElMjIlM0UlMEElMjAlMjAlMjAlMjAlMjAlMjAlMjAlMjAlMjAlMjAlMjAlM0NpbnB1dCUyMGRhdGEtaWQlM0QlMjJzb3VyY2UlM0FpbnB1dCUyMiUyMHR5cGUlM0QlMjJoaWRkZW4lMjIlMjBuYW1lJTNEJTIyc291cmNlJTIyJTIwdmFsdWUlM0QlMjJFRkQlMjIlM0UlMEElMjAlMjAlMjAlMjAlMjAlMjAlMjAlMjAlMjAlMjAlMjAlM0NpbnB1dCUyMGRhdGEtaWQlM0QlMjJyZXF1aXJlZCUzQWlucHV0JTIyJTIwdHlwZSUzRCUyMmhpZGRlbiUyMiUyMG5hbWUlM0QlMjJyZXF1aXJlZCUyMiUyMHZhbHVlJTNEJTIybGlzdCUyQ2VtYWlsJTIyJTNFJTBBJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTNDaW5wdXQlMjBkYXRhLWlkJTNEJTIydXJsJTNBaW5wdXQlMjIlMjB0eXBlJTNEJTIyaGlkZGVuJTIyJTIwbmFtZSUzRCUyMnVybCUyMiUyMHZhbHVlJTNEJTIyJTIyJTNFJTBBJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTNDcCUyMGRhdGEtaWQlM0QlMjJFbWFpbCUyMEFkZHJlc3MlM0FwJTIyJTIwJTNFJTNDbGFiZWwlMjBkYXRhLWlkJTNEJTIyRW1haWwlMjBBZGRyZXNzJTNBbGFiZWwlMjIlMjBkYXRhLW5hbWUlM0QlMjJlbWFpbCUyMiUyMGNsYXNzJTNEJTIyY3RjdC1mb3JtLXJlcXVpcmVkJTIyJTNFRW1haWwlMjBBZGRyZXNzJTNDJTJGbGFiZWwlM0UlMjAlM0NpbnB1dCUyMGRhdGEtaWQlM0QlMjJFbWFpbCUyMEFkZHJlc3MlM0FpbnB1dCUyMiUyMHR5cGUlM0QlMjJ0ZXh0JTIyJTIwbmFtZSUzRCUyMmVtYWlsJTIyJTIwdmFsdWUlM0QlMjIlMjIlMjBtYXhsZW5ndGglM0QlMjI4MCUyMiUzRSUzQyUyRnAlM0UlMEElMjAlMjAlMjAlMjAlMjAlMjAlMjAlMjAlMjAlMjAlMjAlM0NwJTIwZGF0YS1pZCUzRCUyMkZpcnN0JTIwTmFtZSUzQXAlMjIlMjAlM0UlM0NsYWJlbCUyMGRhdGEtaWQlM0QlMjJGaXJzdCUyME5hbWUlM0FsYWJlbCUyMiUyMGRhdGEtbmFtZSUzRCUyMmZpcnN0X25hbWUlMjIlM0VGaXJzdCUyME5hbWUlM0MlMkZsYWJlbCUzRSUyMCUzQ2lucHV0JTIwZGF0YS1pZCUzRCUyMkZpcnN0JTIwTmFtZSUzQWlucHV0JTIyJTIwdHlwZSUzRCUyMnRleHQlMjIlMjBuYW1lJTNEJTIyZmlyc3RfbmFtZSUyMiUyMHZhbHVlJTNEJTIyJTIyJTIwbWF4bGVuZ3RoJTNEJTIyNTAlMjIlM0UlM0MlMkZwJTNFJTBBJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTNDcCUyMGRhdGEtaWQlM0QlMjJMYXN0JTIwTmFtZSUzQXAlMjIlMjAlM0UlM0NsYWJlbCUyMGRhdGEtaWQlM0QlMjJMYXN0JTIwTmFtZSUzQWxhYmVsJTIyJTIwZGF0YS1uYW1lJTNEJTIybGFzdF9uYW1lJTIyJTNFTGFzdCUyME5hbWUlM0MlMkZsYWJlbCUzRSUyMCUzQ2lucHV0JTIwZGF0YS1pZCUzRCUyMkxhc3QlMjBOYW1lJTNBaW5wdXQlMjIlMjB0eXBlJTNEJTIydGV4dCUyMiUyMG5hbWUlM0QlMjJsYXN0X25hbWUlMjIlMjB2YWx1ZSUzRCUyMiUyMiUyMG1heGxlbmd0aCUzRCUyMjUwJTIyJTNFJTNDJTJGcCUzRSUwQSUzQ2lucHV0JTIwdHlwZSUzRCUyMnN1Ym1pdCUyMiUyMHZhbHVlJTNEJTIyU3VibWl0JTIyJTNFJTBBJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTNDJTIxLS0lMjAlM0NidXR0b24lMjB0eXBlJTNEJTIyc3VibWl0JTIyJTIwY2xhc3MlM0QlMjJCdXR0b24lMjBjdGN0LWJ1dHRvbiUyMEJ1dHRvbi0tYmxvY2slMjBCdXR0b24tc2Vjb25kYXJ5JTIyJTIwZGF0YS1lbmFibGVkJTNEJTIyZW5hYmxlZCUyMiUzRVNpZ24lMjBVcCUzQyUyRmJ1dHRvbiUzRSUyMC0tJTNFJTBBJTIwJTIwJTIwJTIwJTIwJTIwJTIwJTNDJTJGZm9ybSUzRSUwQSUyMCUyMCUyMCUzQyUyRmRpdiUzRSUwQSUzQyUyRmRpdiUzRSUwQSUzQ3NjcmlwdCUyMHR5cGUlM0QlMjd0ZXh0JTJGamF2YXNjcmlwdCUyNyUzRSUwQSUyMCUyMCUyMHZhciUyMGxvY2FsaXplZEVyck1hcCUyMCUzRCUyMCU3QiU3RCUzQiUwQSUyMCUyMCUyMGxvY2FsaXplZEVyck1hcCU1QiUyN3JlcXVpcmVkJTI3JTVEJTIwJTNEJTIwJTA5JTA5JTI3VGhpcyUyMGZpZWxkJTIwaXMlMjByZXF1aXJlZC4lMjclM0IlMEElMjAlMjAlMjBsb2NhbGl6ZWRFcnJNYXAlNUIlMjdjYSUyNyU1RCUyMCUzRCUyMCUwOSUwOSUwOSUyN0FuJTIwdW5leHBlY3RlZCUyMGVycm9yJTIwb2NjdXJyZWQlMjB3aGlsZSUyMGF0dGVtcHRpbmclMjB0byUyMHNlbmQlMjBlbWFpbC4lMjclM0IlMEElMjAlMjAlMjBsb2NhbGl6ZWRFcnJNYXAlNUIlMjdlbWFpbCUyNyU1RCUyMCUzRCUyMCUwOSUwOSUwOSUyN1BsZWFzZSUyMGVudGVyJTIweW91ciUyMGVtYWlsJTIwYWRkcmVzcyUyMGluJTIwbmFtZSU0MGVtYWlsLmNvbSUyMGZvcm1hdC4lMjclM0IlMEElMjAlMjAlMjBsb2NhbGl6ZWRFcnJNYXAlNUIlMjdiaXJ0aGRheSUyNyU1RCUyMCUzRCUyMCUwOSUwOSUyN1BsZWFzZSUyMGVudGVyJTIwYmlydGhkYXklMjBpbiUyME1NJTJGREQlMjBmb3JtYXQuJTI3JTNCJTBBJTIwJTIwJTIwbG9jYWxpemVkRXJyTWFwJTVCJTI3YW5uaXZlcnNhcnklMjclNUQlMjAlM0QlMjAlMDklMjdQbGVhc2UlMjBlbnRlciUyMGFubml2ZXJzYXJ5JTIwaW4lMjBNTSUyRkREJTJGWVlZWSUyMGZvcm1hdC4lMjclM0IlMEElMjAlMjAlMjBsb2NhbGl6ZWRFcnJNYXAlNUIlMjdjdXN0b21fZGF0ZSUyNyU1RCUyMCUzRCUyMCUwOSUyN1BsZWFzZSUyMGVudGVyJTIwdGhpcyUyMGRhdGUlMjBpbiUyME1NJTJGREQlMkZZWVlZJTIwZm9ybWF0LiUyNyUzQiUwQSUyMCUyMCUyMGxvY2FsaXplZEVyck1hcCU1QiUyN2xpc3QlMjclNUQlMjAlM0QlMjAlMDklMDklMDklMjdQbGVhc2UlMjBzZWxlY3QlMjBhdCUyMGxlYXN0JTIwb25lJTIwZW1haWwlMjBsaXN0LiUyNyUzQiUwQSUyMCUyMCUyMGxvY2FsaXplZEVyck1hcCU1QiUyN2dlbmVyaWMlMjclNUQlMjAlM0QlMjAlMDklMDklMjdUaGlzJTIwZmllbGQlMjBpcyUyMGludmFsaWQuJTI3JTNCJTBBJTIwJTIwJTIwbG9jYWxpemVkRXJyTWFwJTVCJTI3c2hhcmVkJTI3JTVEJTIwJTNEJTIwJTA5JTA5JTI3U29ycnklMkMlMjB3ZSUyMGNvdWxkJTIwbm90JTIwY29tcGxldGUlMjB5b3VyJTIwc2lnbi11cC4lMjBQbGVhc2UlMjBjb250YWN0JTIwdXMlMjB0byUyMHJlc29sdmUlMjB0aGlzLiUyNyUzQiUwQSUyMCUyMCUyMGxvY2FsaXplZEVyck1hcCU1QiUyN3N0YXRlX21pc21hdGNoJTI3JTVEJTIwJTNEJTIwJTI3TWlzbWF0Y2hlZCUyMFN0YXRlJTJGUHJvdmluY2UlMjBhbmQlMjBDb3VudHJ5LiUyNyUzQiUwQSUwOWxvY2FsaXplZEVyck1hcCU1QiUyN3N0YXRlX3Byb3ZpbmNlJTI3JTVEJTIwJTNEJTIwJTI3U2VsZWN0JTIwYSUyMHN0YXRlJTJGcHJvdmluY2UlMjclM0IlMEElMjAlMjAlMjBsb2NhbGl6ZWRFcnJNYXAlNUIlMjdzZWxlY3Rjb3VudHJ5JTI3JTVEJTIwJTNEJTIwJTA5JTI3U2VsZWN0JTIwYSUyMGNvdW50cnklMjclM0IlMEElMjAlMjAlMjB2YXIlMjBwb3N0VVJMJTIwJTNEJTIwJTI3aHR0cHMlM0ElMkYlMkZ2aXNpdG9yMi5jb25zdGFudGNvbnRhY3QuY29tJTJGYXBpJTJGc2lnbnVwJTI3JTNCJTBBJTNDJTJGc2NyaXB0JTNFJTBBJTNDc2NyaXB0JTIwdHlwZSUzRCUyN3RleHQlMkZqYXZhc2NyaXB0JTI3JTIwc3JjJTNEJTI3aHR0cHMlM0ElMkYlMkZzdGF0aWMuY3RjdGNkbi5jb20lMkZoJTJGY29udGFjdHMtZW1iZWRkZWQtc2lnbnVwLWFzc2V0cyUyRjEuMC4yJTJGanMlMkZzaWdudXAtZm9ybS5qcyUyNyUzRSUzQyUyRnNjcmlwdCUzRSUwQSUzQyUyMS0tRW5kJTIwQ1RDVCUyMFNpZ24tVXAlMjBGb3JtLS0lM0U=...

Cosmetics & Personal Care

August 24, 2016
European legislation and US research activities towards ‘21st century toxicology’ are setting the standards for animal-free toxicological assessments and the cosmetics industry has been the first to feel the effects. As per EU Regulation 1223/2009, animal testing of cosmetic ingredients and finished products is currently banned in the EU, as is the marketing of finished cosmetic products and the ingredients contained therein which were tested on animals for cosmetic safety assessment purposes. These bans hold irrespective of the availability of validated...

Webinar: Regulatory Initiatives for New Approaches to Traditional Toxicity Testing

July 1, 2016
This one-hour webinar features presentations by Dr. Jennifer McLain, Deputy Director, Office of Pesticide Programs, Antimicrobial Division and IIVS CEO Dr. Rodger Curren. Dr. McLain discusses the EPA OPP's plans to reduce the use of animal testing in acute toxicity testing and provides suggestions on how stakeholders and EPA can cooperate to advance animal reduction goals. Dr. Curren then talks about IIVS' activities in developing the current non-animal strategy for eye irritation, and recent investigations into in vitro methods to...

Momentum Grows for Modernizing the US EPA “Six-pack” Battery of Acute Toxicity Tests

January 13, 2016
In the current global regulatory climate favorable for further advancement of in vitro test methods, the US Environmental Protection Agency (US EPA) is driving significant efforts in the United States. The latest endeavor targets the modernization of the battery of acute toxicity tests classically known as the “six-pack” (Fig. 1a). Known for many years as an “all in vivo” testing strategy for products registered under the Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA), the six-pack has recently been revised to include...