IIVS | KeratinoSens (OECD 442D)
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KeratinoSens (OECD 442D)

The KeratinoSensTM assay is a cell-based reporter gene assay which identifies skin sensitizers by measuring the induction of luciferase under the control of the antioxidant response element (ARE) derived from the human AKR1C2 gene. In the proposed Adverse Outcome Pathway (AOP) leading to skin sensitization, this method addresses the second key event, gene expression in keratinocytes associated with the antioxidant/electrophile response element (ARE)-dependent pathway. The IIVS laboratory participated in the validation studies led by Givaudan to assess the KeratinoSensTM assay which demonstrated that the assay was transferable, reproducible, and predictive as compared to the historical animal data. Following extensive review of the data by EURL ECVAM, the assay was endorsed by ECVAM, and the OECD followed with a test guideline (OECD 442D).

Cells are seeded into 96-well plates for use in the KeratinoSens assay for skin sensitization.
Cells are assessed for luciferase induction.