Similar to the KeratinoSens assay (OECD 442D), also offered by IIVS, LuSens addresses the cellular event of Keratinocyte activation, the second key event that triggers the induction of skin sensitization. It is a luciferase-assay bioassay performed in a human keratinocyte cell line. The approach is based on evaluating the upregulation of a gene involved in the induction of the sensitization response.
IIVS participated in an inter-laboratory study led by BASF to assess the transferability, reproducibility, and accuracy of the method. The results demonstrated an accuracy of greater than 80% in identifying skin sensitizers. The study was published in Toxicology in Vitro in 2016.
- Rangefinder assay to ensure appropriate concentrations are tested
- Cytotoxicity assessment included
- Two independent replicates performed
- Suitable for use in a “Weight of Evidence” approach, in conjunction with other in vitro assays
Please contact IIVS for more information on the LuSens assay and how it may be suitable for your organization’s testing needs.