The CAMVA is an in vitro assay that measures the presence and degree of vascular effect to the chorioallantoic membrane (CAM) of 10-day fertilized eggs following exposure to test article.
After exposure, the CAM is examined visually for vascular hemorrhaging, capillary injection and/or ghost vessels at the test material application site. The concentration that elicits a positive response in 50% of the treated CAM specimens (RC50) indicates ocular irritation potential of a test material. Exposed CAM can also be evaluated for efficacy endpoints, such as vascular constriction.
