The Indigo Biosciences Nuclear Receptor (NR) assays offer a fast and accurate way to screen candidate chemicals or ingredients for potential interactions that can disrupt normal biological processes, by binding to receptors on the nuclear membrane. Discover agonist and antagonist potentials of known and new substances, whether you are running high-throughput on-target drug discovery or assessing potentially toxic off-target interactions.
Indigo Biosciences Nuclear Receptor
Go from test initiation > luminosity with our Tecan© Luminometer > receiving reliable data analysis in a matter of days, all in a fully-compliant Good Lab Practices (GLP) and Good In Vitro Method Practices (GIVIMP) certified environment.
The Indigo Biosciences NR assays are cell-based trans-activation assays that utilize firefly luciferase reporter gene technology to quantify any functional activity, either agonist or antagonist, that the test substances may exert against the nuclear receptors. Luciferase gene expression occurs after ligand-bound nuclear receptor undergoes nuclear translocation, DNA binding, recruitment and assembly of the co-activators and accessory factors required to form a functional transcription complex, culminating in expression of the target gene. Therefore, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measurement of the changes in receptor activity. In other words, the level of light emission (Relative Light Units, RLU) directly correlates with the strength of the interaction. By incorporating the Live Cell Multiplex (LCM), we can confirm the primary NR assay data by evaluating potential mitogenic, cytostatic or cytotoxic effects on the reporter cells.
Assay Features:
- Large library of nuclear receptor assays
- Test for both agonist and antagonist potential
- Evaluate a compounds’ efficacy, potency, and selectivity
- Results available in days
- Provide “mode of action” information
Common Receptors of interest:
- Androgen Receptor (AR)
- Estrogen Receptor (ERα)
- Thyroid Hormone Receptor Alpha (TRα)
- Peroxisome Proliferator-Activated Receptor Alpha (PPARα)
- Glucocorticoid Receptor (GR)
To learn more about all of the additional receptors we support, contact us.
Industries of Interest:
- Researchers (Government/Academic) – Learn functional activity of receptors in disease states
- Drug Development – Reduce time and money spent on early drug discovery
- Environmental Monitoring – Bioanalytical analysis of water reuse
- Industrial
- Consumer Goods