Reconstructed Skin Micronucleus (RSMN) Using the EpiDerm™ Reconstructed Human Epidermis Model
RSMN is used to evaluate the genotoxicity potential of the test article to the EpiDerm™ Skin Model. Genotoxicity potential is determined by assessing whether the micronucleus frequency in tissues exposed to the test article showed a statistically significant increase relative to the tissues exposed to the solvent (negative) control.
Evaluate the genotoxic potential of new ingredients and chemicals at exposure-relevant doses
- RSMN determines the skin genotoxicity/mutagenicity potential of a test substance by determining the relative increase in the induction of micronuclei in test substance- treated tissues compared to negative control-treated tissues
- Reconstructed human epidermal tissues may more accurately model the complexity of skin absorption, human epidermal metabolism, and tissue-specific responses than traditional monolayer culture systems
- Complex test formulations may be applied in a biologically-relevant fashion directly onto the stratum corneum
- Protocols allow for 48 and 72 hour exposures on the metabolically active tissues with no exogenous source of metabolic activation (S9)
- Scientific Committee on Consumer Safety (SCCS) of European Commission acknowledges potential uses of RSMN as a 2nd tier genotoxicity testing