Kinetic Direct Peptide Reactivity Assay (kDPRA)

Building on the original DPRA (OECD TG 442C), this test assesses multiple substance concentrations and time points to provide quantitative reaction data. This information can assist in determining potency and has the potential to classify substances into UN GHS sub-categories 1A and 1B .

Similar to the DPRA assay, kDPRA examines the reactivity of test materials with nucleophilic peptides. High levels of conjugation are indicative of a potential sensitizer.

IIVS participated in the validation of the kinetic DPRA with test method developers BASF and Givaudan in 2018 and is currently being evaluated for a Test Guideline by the OECD.

IIVS offers the kinetic DPRA as a standalone assay or as part of a battery of services with ARE-Nrf2 Luciferase Test Methods (OECD 442D) and the Human Cell Line Activation Test (OECD442E). IIVS also offers the Direct Peptide Reactivity Activation Assay (OECD 442C) and SENS-IS® – a 3D reconstructed epidermis-based assay that quantifies sensitization potency and evaluates skin irritation in a single assay.

Assay Features

  • Resolution of GHS Category 1A and 1B sensitizers (vs. non-classified)
  • Quick Turnaround
  • Less expensive than most animal-based studies
  • OECD Test Guideline Pending

The Tecan Infinite® M Nano+ is used to evaluate cysteine conjugation in the kDPRA. This device was generously donated by the Research Institute for Fragrance Materials (RIFM).

Please contact IIVS for more information on the kDPRA assay and how it may be suitable for your organization’s testing needs.