The ocular irritation potential of formulations, products, ingredients, and chemicals can be evaluated using in vitro reconstructed human corneal epithelium (RhCE) models, such as the EpiOcular™ (MatTek Corp.) and SkinEthic HCE (EPISKIN) organotypic 3-D tissue constructs. Whether evaluating ultra-mild cosmetics and personal care products, or rank ordering the irritation potential of candidate formulations and ingredients, we can provide custom Ocular Screening protocols to best meet your testing goals.
The Ocular Screening protocols use a time-to-toxicity procedure to determine the test material exposure time that induces a 50% reduction in viability, relative to the negative control (ET50 or t50). Since the mechanistic basis for eye irritation involves corneal penetration and cellular/tissue damage, the measurement of cell viability is highly relevant. Therefore, the viability of the RhCE tissues is determined by the NAD(P)H-dependent microsomal enzyme reduction (and to a lesser extent by the succinate dehydrogenase reduction) of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide).