Non Animal Testing, Alternative Test Methods, In Vitro Toxicology, IIVS | Assays AGAR Overlay
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Medical Devices

August 24, 2016
IIVS offers in vitro cytotoxicity assay using Modified Eagles Medium (MEM) elution and agar overlay for medical devices.  These in vitro biological reactivity assays, MEM Elution and Agar Diffusion, meet the United States Pharmacopoeia (USP) and International Organization for Standardization (ISO) 10993 requirements. Clients include medical devices, consumer products, and cosmetic companies with products defined as medical devices....

AGAR Overlay

August 22, 2016
The agar overlay assay or agar diffusion test is an in vitro cytotoxicity assay designed to show the presence of toxic material eluted from a test sample as it affects L929 cells cultured in the presence of the test article. Test articles are placed onto the agar surface overlaid onto a L929 cell monolayer preventing direct damage while allowing diffusion of leachable chemicals from the sample. After treatment, the cells are exposed to neutral red dye to differentiate between the viable...