AGAR Overlay

August 22, 2016
The agar overlay assay or agar diffusion test is an in vitro cytotoxicity assay designed to show the presence of toxic material eluted from a test sample as it affects L929 cells cultured in the presence of the test article. Test articles are placed onto the agar surface overlaid onto a L929 cell monolayer preventing direct damage while allowing diffusion of leachable chemicals from the sample. After treatment, the cells are exposed to neutral red dye to differentiate between the viable...