Archive
Webinar: How GLPs Enhance the Quality of Regulated and Non-Regulated Toxicology
October 18, 2016This one-hour webinar, led by IIVS Director of Quality and Compliance, introduces some of the concepts of Good Laboratory Practices (GLPs) designed to promote study and data integrity within an in vitro toxicology framework. Applying these concepts within your own laboratory should aid in production of robust, repeatable studies. View Slides...
Reconstructed Human Epidermis (RhE) Models
August 24, 2016The 3-D Phototoxicity assay uses 3-D reconstructed human epidermal (RhE) tissue constructs to evaluate the dermal phototoxicity potential of a test material. Toxicity is determined by measuring cytotoxicity [based on the relative conversion of MTT (3-[4,5 - dimethylthiazol-2-yl] - 2,5 - diphenyltetrazolium bromide)] in cultures treated with the test material in the presence or absence of UVA light. An advantage of using 3-D tissues to evaluate phototoxicity potential is that, unlike the 96-well phototoxicity assay, test materials are applied topically, so solids, undiluted...
3T3 Neutral Red Uptake (OECD 432)
August 24, 2016The 3T3 Neutral Red Uptake (NRU) Phototoxicity assay is a 96-well cytotoxicity-based assay that utilizes normal BALB/c 3T3 mouse fibroblasts to measure the concentration-dependent reduction in neutral red uptake by the cells after exposure to a test material either in the presence or absence of UVA light. Duplicate 96-well monolayers of 3T3 fibroblasts are exposed to serial dilutions of a test material. One of the plates is exposed to 5 J/cm2 UVA while the other plate is kept in the...
Medical Devices
August 24, 2016IIVS offers in vitro cytotoxicity assay using Modified Eagles Medium (MEM) elution and agar overlay for medical devices. These in vitro biological reactivity assays, MEM Elution and Agar Diffusion, meet the United States Pharmacopoeia (USP) and International Organization for Standardization (ISO) 10993 requirements. Clients include medical devices, consumer products, and cosmetic companies with products defined as medical devices....
MEM Elution
August 21, 2016The MEM elution assay or elution test is an in vitro cytotoxicity assay designed to show the presence of toxic material eluted from a test sample as it affects L929 cells cultured in the presence of the extract. Extracts of test articles are applied to L929 cells. After treatment, the cells are exposed to neutral red dye to differentiate between the viable and stressed or lysed cells. The degree of irritancy is determined microscopically in the percentage of cells deformed and...