August 24, 2016
The 3-D Phototoxicity assay uses reconstructed human epidermal (RhE) tissue constructs to evaluate the dermal phototoxicity potential of a test material. Toxicity is determined by measuring cytotoxicity [based on the relative conversion of MTT (3-[4,5 – dimethylthiazol-2-yl] – 2,5 – diphenyltetrazolium bromide)] in cultures treated with the test material in the presence and absence of UVA light.
An advantage of using 3-D tissues to evaluate phototoxicity potential is that, unlike the 96-well phototoxicity assay, test materials are applied topically. As such, solids, undiluted...
3T3 Neutral Red Uptake | MEM Elution | NHEK & 3T3 | Phototoxicity | Tissue Models | UV Induced Toxicity | photoxicity | Reconstructed human Epidermis | RhE
August 21, 2016The MEM elution assay or elution test is an in vitro cytotoxicity assay designed to show the presence of toxic material eluted from a test sample as it affects L929 cells cultured in the presence of the extract.
Extracts of test articles are applied to L929 cells. After treatment, the cells are exposed to neutral red dye to differentiate between the viable and stressed or lysed cells. The degree of irritancy is determined microscopically in the percentage of cells deformed and...
MEM Elution | Medical Devices