August 24, 2016
The 3-D Phototoxicity assay uses reconstructed human epidermal (RhE) tissue constructs to evaluate the dermal phototoxicity potential of a test material. Toxicity is determined by measuring cytotoxicity [based on the relative conversion of MTT (3-[4,5 – dimethylthiazol-2-yl] – 2,5 – diphenyltetrazolium bromide)] in cultures treated with the test material in the presence and absence of UVA light.
An advantage of using 3-D tissues to evaluate phototoxicity potential is that, unlike the 96-well phototoxicity assay, test materials are applied topically. As such, solids, undiluted...
3T3 Neutral Red Uptake | MEM Elution | NHEK & 3T3 | Phototoxicity | Tissue Models | UV Induced Toxicity | photoxicity | Reconstructed human Epidermis | RhE