Archive
In Vitro Assessment of Skin Irritation Potential of Surfactant-based Formulations by Using a 3-D Skin Reconstructed Tissue Model and Cytokine Response
January 24, 2017The personal care industry is focused on developing safe, more efficacious, and increasingly milder products, that are routinely undergoing preclinical and clinical testing before becoming available for consumer use on skin. In vitro systems based on skin reconstructed equivalents are now established for the preclinical assessment of product irritation potential and as alternative testing methods to the classic Draize rabbit skin irritation test. We have used the 3-D EpiDerm™ model system to evaluate tissue viability and primary cytokine interleukin-1α release...
Webinar: How GLPs Enhance the Quality of Regulated and Non-Regulated Toxicology
October 18, 2016This one-hour webinar, led by IIVS Director of Quality and Compliance, introduces some of the concepts of Good Laboratory Practices (GLPs) designed to promote study and data integrity within an in vitro toxicology framework. Applying these concepts within your own laboratory should aid in production of robust, repeatable studies. View Slides...
Phototoxicity in Reconstructed Human Epidermal (RhE) Models
August 24, 2016The 3-D Phototoxicity assay uses reconstructed human epidermal (RhE) tissue constructs to evaluate the dermal phototoxicity potential of a test material. Toxicity is determined by measuring cytotoxicity [based on the relative conversion of MTT (3-[4,5 – dimethylthiazol-2-yl] – 2,5 – diphenyltetrazolium bromide)] in cultures treated with the test material in the presence and absence of UVA light. An advantage of using 3-D tissues to evaluate phototoxicity potential is that, unlike the 96-well phototoxicity assay, test materials are applied topically. As such, solids, undiluted...
3T3 Phototoxicity (OECD 432)
August 24, 2016The 3T3 Neutral Red Uptake (NRU) Phototoxicity assay is a 96-well cytotoxicity-based assay that utilizes BALB/c 3T3 mouse fibroblasts to measure the concentration-dependent reduction in neutral red uptake by the cells after exposure to a test material in the presence and absence of UVA light. Duplicate 96-well plates containing a monolayer of 3T3 cells are exposed to serial dilutions of a test material. One of the plates is exposed to 5 J/cm2 of UVA while the other plate is kept...
Webinar: In Vitro 3T3 Phototoxicity Assay Practical Experiences Assay Optimization and Challenges
April 9, 2015...
Assessing increased sensitivity and variability issues in an established in vitro phototoxicity testing program
November 1, 2014Source: 2014 ASCCT Meeting...
Screening of Cosmetics Ingredients for Phototoxic Potential Using the In Vitro 3T3 Neutral Red Uptake Phototoxicity Test
March 12, 2013Source: Presented at the 2013 Society of Toxicology Annual Meeting...