Non Animal Testing, Alternative Test Methods, In Vitro Toxicology, IIVS | Assays Trans-Epithial Permeability

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Archive

Webinar: How GLPs Enhance the Quality of Regulated and Non-Regulated Toxicology

October 18, 2016
This one-hour webinar, led by IIVS Director of Quality and Compliance, introduces some of the concepts of Good Laboratory Practices (GLPs) designed to promote study and data integrity within an in vitro toxicology framework. Applying these concepts within your own laboratory should aid in production of robust, repeatable studies. View Slides... 3T3 Neutral Red Uptake | Acute Respiratory Toxicity | AGAR Overlay | Assessing Corneal Recovery | BCOP | Bronchial/Tracheal | CAMVA | Chronic Respiratory Toxicity | Ciliary Beat Frequency | Corneal Recovery Modeling

Cytosensor Microphysiometer

August 24, 2016
The Cytosensor Microphysiometer assay is an in vitro cellular toxicity test used to evaluate ocular irritancy. A microphysiometer is used to detect and monitor the extracellular changes in pH in L929 (mouse fibroblast) cells after exposure to a test material. Changes in the pH are caused by variations in the metabolic rate, measured indirectly as a function of changes in extracellular acidification. Metabolism in living cells is tightly coupled to cellular ATP usage and extracellular release of acidic byproducts such as protons, lactic... Assessing Corneal Recovery | BCOP | CAMVA | Corneal Recovery Modeling | Eye Irritation Test | NociOcular Eye Sting | Ocular Irritection | Ocular Screening | Short Time Exposure | Trans-Epithial Permeability

Trans-Epithelial Permeability/Fluorescein Leakage (OECD 460)

August 24, 2016
The Trans-Epithelial Permeability (TEP) Assay, is a cell-based assay used to evaluate the potential ocular irritancy of test chemicals by measuring the permeability of sodium fluorescein (or fluorescein leakage) through a confluent monolayer of Madin-Darby Canine Kidney (MDCK) cells. The MDCK cell line is used since it forms tight junctions in a confluent monolayer similar to those formed in the outermost corneal and conjunctival epithelial layers. The disruption of the tight junctions by chemicals is one of the early events typical... Trans-Epithial Permeability

Webinar: Serious Eye Damage and Eye Irritation

Using TRPV1 Channel Activity to Predict the Eye Stinging Potential of Baby Cleansers