Archive
Evaluation of TRPV1 Activity to Assess the Eye Stinging Potential of Cosmetic Formulations
October 17, 2019The Transient Receptor Potential Vanilloid type 1 (TRPV1) receptor is one of the most well characterized pain-inducing receptors and has been recently identified as a valuable tool to predict eye stinging potential of surfactant based formulations. In this study we sought to predict eye stinging of nonsurfactant based cosmetic formulations by studying TRPV1 activity using the NociOcular assay. In the NociOcular assay, TRPV1 expressing neuroblastoma cells are exposed to test substance and TRPV1 activity is measured by acute increases in...
Strategies for Eye Irritation Testing
September 17, 2018Determination of ocular irritation potential is a key toxicological endpoint required to support chemical and raw material characterization and industrial hygiene, to guide product development, and to achieve final product safety standards. IIVS possesses extensive experience with numerous test methods and testing strategies for commercial, industrial and regulatory applications. From standard regulatory hazard assessments to providing information on the degree of ocular irritation, to discriminating among extremely mild eye area products, IIVS’ Study Directors take the time to understand your...
Webinar: How GLPs Enhance the Quality of Regulated and Non-Regulated Toxicology
October 18, 2016This one-hour webinar, led by IIVS Director of Quality and Compliance, introduces some of the concepts of Good Laboratory Practices (GLPs) designed to promote study and data integrity within an in vitro toxicology framework. Applying these concepts within your own laboratory should aid in production of robust, repeatable studies. View Slides...
Ocular Irritection (OECD 496)
August 24, 2016The Ocular Irritection Assay is an in chemico eye irritation assay that detects, ranks, and predicts the corneal irritation potential of a test material. The assay assesses changes to the reagent solution (containing proteins, glycoproteins, lipids), which mimics the denaturation and disruption that occurs in corneal proteins in vivo.The test article is applied to a membrane disc that controls the delivery to the reagent solution. The changes in the protein structure are measured by optical density readings in the reagent...
Short Time Exposure (STE, OECD 491)
August 24, 2016The Short Time Exposure (STE) assay, developed by Kao Corporation (Japan), is an in vitro assay used to assess acute eye irritation potential as an alternative to the traditional in vivo Draize test. The test method evaluates the cytotoxicity induced by a series of test chemical dilutions in a monolayer of rabbit corneal fibroblasts (Statens Seruminstitut Rabbit Cornea – SIRC) after a single five-minute exposure. Two prediction models were initially developed for the STE assay — one categorizes the test material as...
Bovine Corneal Opacity and Permeability (BCOP, OECD 437) with Optional Histology
August 24, 2016The BCOP (Bovine Corneal Opacity and Permeability) assay is an in vitro eye irritation test method developed by Gautheron et al. (1992), which uses living bovine corneal tissue, obtained as a by-product from abattoirs, to evaluate the potential ocular irritancy of a test article. Types of injury caused by exposure to the test article are quantitatively measured by changes in opacity and permeability to fluorescein. The BCOP assay allows for the investigation of the mechanism of the damage caused. Corneal opacity...
Eye Irritation Time-to-Toxicity Test using Reconstructed Human Corneal Epithelium (RhCE) Models
August 24, 2016The ocular irritation potential of formulations, products, ingredients, and chemicals can be evaluated using in vitro reconstructed human corneal epithelium (RhCE) models, such as the EpiOcular™ (MatTek Corp.) and SkinEthic HCE (EPISKIN) organotypic 3-D tissue constructs. Whether evaluating ultra-mild cosmetics and personal care products, or rank ordering the irritation potential of candidate formulations and ingredients, we can provide custom Ocular Screening protocols to best meet your testing goals. The Ocular Screening protocols use a time-to-toxicity procedure to determine the test material...
Improving Sensitivity of the NociOcular Assay to Evaluate Ion Channel Activation by Acidic Cosmetic Ingredients
April 1, 2016Source: Pan-American Conference on Alternatives ...
Evaluation of TRPV1 Activity to Assess the Eye Stinging Potential of Cosmetic Formulations
March 23, 2015Source: 2015 Society of Toxicology Meeting...