Documents & Resources

Observations on the Use of the Bovine Corneal Opacity and Permeability (BCOP) Assay to Evaluate the Eye Irritation Potential of Prototype Cosmetic Formulations Containing Salicylic Acid, Glycolic Acid and Ethanol

Source: World Congress 9

Webinar: Reconstructed Human Skin Micronucleus Assay (RSMN): A Novel Micronucleus Assay in a 3D Human Skin Model

TIERED TESTING STRATEGY USING VALIDATED IN VITRO ASSAYS FOR THE ASSESSMENT OF SKIN AND EYE CORROSION/IRRITATION OF PHARMACEUTICAL INTERMEDIATES

Source: SOT 2014

Testing Framework for Prediction of Ocular Irritation Using the Bovine Corneal Opacity and Permeability (BCOP) Assay and Chorioallantoic Membrane Vascular Assay (CAMVA)

Source: SOT 2014

Investigations on Reducing Ocular Irritation Associated with Harsh Ingredients by Altering Physicochemical Properties of the Formulation

Source: SOT 2014

IN VITRO SAFETY PROFILE OF PERSONAL CARE PRODUCTS – USE OF AN IN VITRO TESTING PLATFORM BASED ON A RECONSTRUCTED VAGINAL TISSUE MODEL

Source: SOT 2014

Webinar Slides: The NociOcular Assay: A Novel Assay Looking at TRPV1 Channel Activity for Eye Sting Predictions Webinar

Validation and Application of the KeratinoSens Assay, a Novel In Vitro Skin Sensitization Assay

Predicting Ocular Irritation of Surfactants Using the Bovine Corneal Opacity and Permeability Assay

The Bovine Corneal Opacity and Permeability (BCOP) assay is an ex vivo test used to evaluate ocular irritation. According to the OECD Test Guideline (TG) 437, the BCOP assay can be used to identify chemicals which induce severe/corrosive eye irritation and those that do not require classification. However, BCOP has historically under-predicted certain anionic surfactants, when tested according to the standard liquid protocol. Since neither the basis for selecting the appropriate surfactant test methods, nor the justification for modifications are clearly presented in TG 437 or GD No. 160, we present on the testing of a few common surfactant ingredients, including sodium lauryl sulfate (SLS), Triton X-100, and benzalkonium chloride, and surfactant based formulations in the BCOP assay using standard and modified dilutions and exposures to elucidate the impact of these variables on eye irritation prediction.

Using TRPV1 Channel Activity to Predict the Eye Stinging Potential of Baby Cleansers

Source: JSAAE meeting