Non Animal Testing, Alternative Test Methods, In Vitro Toxicology, IIVS | Industries Agro/Petrochemical
60
archive,paged,tax-industry,term-agro-petrochemical,term-60,paged-2,ajax_fade,page_not_loaded,,qode_grid_1300,footer_responsive_adv,hide_top_bar_on_mobile_header,qode-content-sidebar-responsive,qode-child-theme-ver-,qode-theme-ver-11.0,qode-theme-bridge,wpb-js-composer js-comp-ver-4.11.2.1,vc_responsive

Archive

Addressing the Assignment of US EPA Hazard Categories for Dermal Safety by a Revised Prediction Model of the Validated In Vitro Skin Irritation Test (OECD TG 439)

March 30, 2017
One of the current goals of the United States Environmental Protection Agency Office of Pesticide Programs (US EPA OPP) is to replace by non-animal testing methods as many of the endpoints of the battery of acute toxicity tests known as the “6-pack” as possible. One of the “6-pack” tests is the Draize rabbit test for dermal irritation. We investigated whether the validated in vitro Skin Irritation Test (SIT, OECD TG 439) can be used to determine US EPA OPP dermal...

Webinar: How GLPs Enhance the Quality of Regulated and Non-Regulated Toxicology

October 18, 2016
This one-hour webinar, led by IIVS Director of Quality and Compliance, introduces some of the concepts of Good Laboratory Practices (GLPs) designed to promote study and data integrity within an in vitro toxicology framework. Applying these concepts within your own laboratory should aid in production of robust, repeatable studies. View Slides...

KeratinoSens (OECD 442D)

August 24, 2016
The KeratinoSensTM assay is a cell-based reporter gene assay that models the second key event in the Adverse Outcome Pathway for Dermal Sensitization, namely keratinocyte activation.  This approach measures the induction of luciferase under the control of the antioxidant response element (ARE) derived from the human AKR1C2 gene, and can be used in a Weight of Evidence approach as an alternative to animal testing methods for the purpose of hazard identification. IIVS has participated in validation studies led by the assay developer...

Bovine Corneal Opacity and Permeability (OECD 437) & Histology

August 24, 2016
The BCOP (Bovine Corneal Opacity and Permeability) assay is an in vitro eye irritation test method developed by Gautheron et al. (1992), which uses living bovine corneal tissue, obtained as a by-product from abattoirs, to evaluate the potential ocular irritancy of a test article. Types of injury caused by exposure to the test article are quantitatively measured by changes in opacity and permeability to fluorescein. The BCOP assay allows for the investigation of the mechanism of the damage caused. Corneal opacity...

Eye Irritation Time-to-Toxicity

August 24, 2016
The ocular irritation potential of formulations, products, ingredients, and chemicals can be evaluated using in vitro reconstructed human corneal epithelium (RhCE) models, such as the EpiOcular™ (MatTek Corp.) and SkinEthic HCE (EPISKIN) organotypic 3-D tissue constructs.  Whether evaluating ultra-mild cosmetics and personal care products, or rank ordering the irritation potential of candidate formulations and ingredients, we can provide custom Ocular Screening protocols to best meet your testing goals. The Ocular Screening protocols use a time-to-toxicity procedure to determine the test material...

Agro/Petrochemical

July 28, 2016
Agricultural chemicals consist of multiple product types including fertilizers, pesticides, and herbicides. As a result, these chemicals can cover a wide range in the irritation spectrum, from materials that are classified as mild irritants to those that are designated as corrosive. Classification is needed for registration through organizations such as the US EPA, REACH, or Brazil ANVISA. Multiple in vitro assays have OECD test guidelines that can be used to provide GHS hazard classifications that may meet necessary testing requirements. Ocular...

Webinar: Regulatory Initiatives for New Approaches to Traditional Toxicity Testing

July 1, 2016
This one-hour webinar features presentations by Dr. Jennifer McLain, Deputy Director, Office of Pesticide Programs, Antimicrobial Division and IIVS CEO Dr. Rodger Curren. Dr. McLain discusses the EPA OPP's plans to reduce the use of animal testing in acute toxicity testing and provides suggestions on how stakeholders and EPA can cooperate to advance animal reduction goals. Dr. Curren then talks about IIVS' activities in developing the current non-animal strategy for eye irritation, and recent investigations into in vitro methods to...

Evaluation of the Validated In Vitro Skin Irritation Test (OECD TG 439) for the Assignment of EPA Hazard Categories

April 1, 2016
Progress within the in vitro toxicology field has made available testing platforms based on reconstructed tissue models that are validated to address the skin corrosion and irritation endpoints. While the validated in vitro assays can be used for the hazard identification of chemicals irritant to skin in accordance with the United Nations (UN) Globally Harmonized System (GHS) for Classification and Labeling of Chemicals, they were not calibrated to address the classification system used by the United States Environmental Protection Agency...