Archive
Webinar: How GLPs Enhance the Quality of Regulated and Non-Regulated Toxicology
October 18, 2016This one-hour webinar, led by IIVS Director of Quality and Compliance, introduces some of the concepts of Good Laboratory Practices (GLPs) designed to promote study and data integrity within an in vitro toxicology framework. Applying these concepts within your own laboratory should aid in production of robust, repeatable studies. View Slides...
In vitro Phototoxicity – Reconstructed Human Epidermis Phototoxicity test (OECD 498)
August 24, 2016The 3-D Phototoxicity assay uses reconstructed human epidermal (RhE) tissue constructs to evaluate the dermal phototoxicity potential of a test material. Toxicity is determined by measuring cytotoxicity [based on the relative conversion of MTT (3-[4,5 – dimethylthiazol-2-yl] – 2,5 – diphenyltetrazolium bromide)] in cultures treated with the test material in the presence and absence of UVA light. An advantage of using 3-D tissues to evaluate phototoxicity potential is that, unlike the 96-well phototoxicity assay, test materials are applied topically. As such, solids, undiluted...
3T3 Phototoxicity (OECD 432)
August 24, 2016The 3T3 Neutral Red Uptake (NRU) Phototoxicity assay is a 96-well cytotoxicity-based assay that utilizes BALB/c 3T3 mouse fibroblasts to measure the concentration-dependent reduction in neutral red uptake by the cells after exposure to a test material in the presence and absence of UVA light. Duplicate 96-well plates containing a monolayer of 3T3 cells are exposed to serial dilutions of a test material. One of the plates is exposed to 5 J/cm2 of UVA while the other plate is kept...
NHEK & 3T3
August 22, 2016The 3T3 Neutral Red Uptake (NRU) Cytotoxicity Assay using a Neutral Red Uptake (NRU) viability endpoint is a 96-well cytotoxicity assay to assess the toxicity potential of a test material. The assay utilizes Balb/c 3T3 mouse fibroblasts to assess the systemic toxicity potential of a test material and may be used to predict in vivo rodent LD50 starting doses for acute oral systemic toxicity. Cytotoxicity caused by the test article is measured by a concentration-dependent reduction in neutral red dye uptake by the...
Neutral Red Uptake
August 21, 2016The Normal Human Epidermal Keratinocytes (NHEKs) Cytotoxicity Assay using a Neutral Red Uptake (NRU) viability endpoint is a 96-well cytotoxicity assay to assess the toxicity potential of a test material. The assay utilizes primary NHEK cells and may be used to predict either human lethal serum concentrations or in vivo rodent LD50 starting doses for acute oral systemic toxicity. Cytotoxicity caused by the test article is measured by a concentration-dependent reduction in neutral red uptake (NRU) by the cells after exposure to a...
Webinar: In Vitro 3T3 Phototoxicity Assay Practical Experiences Assay Optimization and Challenges
April 9, 2015...
Screening of Cosmetics Ingredients for Phototoxic Potential Using the In Vitro 3T3 Neutral Red Uptake Phototoxicity Test
March 12, 2013Source: Presented at the 2013 Society of Toxicology Annual Meeting...
ESTIMATING SYSTEMIC TOXICITY IN VITRO USING AN ADENOSINE TRIPHOSPHATE CYTOTOXICITY (ATP) ASSAY IN NORMAL HUMAN EPIDERMAL KERATINOCYTES
January 1, 2007Source: Presented at the 6th World Congress on Alternatives and Animal Use in the Life Sciences, Tokyo, Japan, August 21-25, 2007...