Non Animal Testing, Alternative Test Methods, In Vitro Toxicology, IIVS | Industries Fragrance & Flavors
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Direct Peptide Reactivity Activation Assay (DPRA, OECD 442C)

August 24, 2016
The DPRA  (OECD 442C) is an in chemico assay that  models the first key event in the skin sensitization Adverse Outcome Pathway (AOP) - skin, protein reactivity. Compounds implicated in causing Allergic Contact Dermatitis (ACD) are generally electrophilic in nature. This assay identifies dermal sensitizers based on their reactivity with synthetic peptides containing the nucleophilic amino acid residues lysine and cysteine. Using LC/UV, conjugation of the test material with the peptides can be analyzed. Trained by the developers of this assay (Procter & Gamble), IIVS...

Eye Irritation Test (EIT, OECD 492)

August 24, 2016
The Eye Irritation Test (EIT) is an OECD-approved in vitro non-animal test method for identifying chemicals and mixtures that may be irritating to the corneal epithelium.  The test method utilizes an in vitro reconstructed human corneal epithelium (RhCE) model (EpiOcular™, MatTek Corp. or HCE, SkinEthic™), in an acute exposure assay to support international regulatory labeling requirements, according to the United Nations Globally Harmonized System of Classification and Labelling of Chemicals (UN GHS). The Eye Irritation Test can be used to support...

Fragrance & Flavors

August 24, 2016
Exposure to fragrance chemicals is most commonly by direct skin contact. Allergic contact dermatitis (ACD) to fragrance ingredients is most often caused by cosmetic products, such as perfumes, deodorants, and aftershave products. Cosmetic fragrance ingredients should be assessed for exposure damage including skin sensitization (ACD), phototoxicity, skin irritation (irritant contact dermatitis), and respiratory irritation and sensitization. At IIVS, we have pre-clinical testing programs to address each of these endpoints to ensure the safety of fragrance 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Webinar: Regulatory Initiatives for New Approaches to Traditional Toxicity Testing

July 1, 2016
This one-hour webinar features presentations by Dr. Jennifer McLain, Deputy Director, Office of Pesticide Programs, Antimicrobial Division and IIVS CEO Dr. Rodger Curren. Dr. McLain discusses the EPA OPP's plans to reduce the use of animal testing in acute toxicity testing and provides suggestions on how stakeholders and EPA can cooperate to advance animal reduction goals. Dr. Curren then talks about IIVS' activities in developing the current non-animal strategy for eye irritation, and recent investigations into in vitro methods to...

Evaluation of New Solvents for the Use in the Multi‐Dose Reconstructed Human EpiDermis (RhE) Phototoxicity Assay

March 17, 2016
The phototoxic potential of test materials after exposure to UVA/visible light is evaluated by the 3T3 Neutral Red Uptake Phototoxicity Assay using Balb/c 3T3 mouse fibroblasts (OECD TG 432). To address challenges related to testing of finished products or materials that are not completely soluble, the Reconstructed Human EpiDermis (RhE) Phototoxicity Assay (INVITTOX Protocol 121) can be used as a stand‐alone or in a tiered approach. Water and sesame oil were recommended solvents for the RhE Assay, however additional solvents...