Archive

21 Nov Video: The 3T3 Neutral Red Uptake Phototoxicity Test — Portuguese

Posted at 04:27h in Uncategorized by Jane Ryan 0 Comments

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This new training video with Portuguese subtitles explores a cell-based method for assessing Phototoxicity — or the potential for chemicals to cause damage after being exposed to light. The method is used widely around the world by many industries, including the cosmetics and pharmaceutical sectors. Disclaimer:...

18 Oct Webinar: How GLPs Enhance the Quality of Regulated and Non-Regulated Toxicology

Posted at 18:45h in Uncategorized by Jane Ryan 0 Comments

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[vc_row css_animation="" row_type="row" use_row_as_full_screen_section="no" type="full_width" angled_section="no" text_align="left" background_image_as_pattern="without_pattern"][vc_column][vc_column_text css=""]Oct 18, 2016 This one-hour webinar, led by IIVS Director of Quality and Compliance, introduces some of the concepts of Good Laboratory Practices (GLPs) designed to promote study and data integrity within an in vitro toxicology framework. Applying these...

18 Oct Advancing Non-animal Testing Methods: First Set of Novel Knowledge Tools Published

Posted at 18:14h in Uncategorized by Jane Ryan 0 Comments

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An adverse outcome pathway, or AOP, is a highly structured way of describing a toxicological process which can lead to an adverse health effect in humans or wildlife, caused by an unsafe exposure to a chemical substance. The first five adverse outcome pathways have now...

06 Sep IIVS Announces Suite of Non-Animal Methods to Reduce Animal Testing for Skin Sensitization

Posted at 02:02h in Uncategorized by Jane Ryan 0 Comments

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GAITHERSBURG, MD – September 6, 2016 – In response to a newly published OECD Test Guideline, IIVS announces the addition of the Human Cell Line Activation Test (h-CLAT, OECD TG 442E) to its suite of non-animal assays to determine skin sensitization. The assessment of skin sensitization...

24 Aug 3T3 Phototoxicity (OECD 432)

Posted at 16:52h in by Jane Ryan 0 Comments

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[vc_row css_animation="" row_type="row" use_row_as_full_screen_section="no" type="full_width" angled_section="no" text_align="left" background_image_as_pattern="without_pattern"][vc_column width="2/3"][vc_column_text]The 3T3 Neutral Red Uptake (NRU) Phototoxicity assay is a 96-well cytotoxicity-based assay that utilizes BALB/c 3T3 mouse fibroblasts to measure the concentration-dependent reduction in neutral red uptake by the cells after exposure to a test material...

24 Aug Human Cell Line Activation Test (h-CLAT, OECD 442E)

Posted at 16:50h in by Jane Ryan 0 Comments

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[vc_row css_animation="" row_type="row" use_row_as_full_screen_section="no" type="full_width" angled_section="no" text_align="left" background_image_as_pattern="without_pattern"][vc_column width="2/3"][vc_column_text]The human Cell Line Activation Test (h-CLAT) is a cell-based assay that identifies skin sensitizers by examining changes in the expression of cell surface markers (CD54 and CD86) implicated in dendritic cell activation, the third key event of the skin sensitization AOP. Following exposure of the THP-1 human monocyte cell line to the test substance, expression levels of CD54 and CD86 are quantified by flow cytometry and compared to controls.

24 Aug Direct Peptide Reactivity Assay (DPRA, OECD 442C)

Posted at 16:50h in by Jane Ryan 0 Comments

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[vc_row css_animation="" row_type="row" use_row_as_full_screen_section="no" type="full_width" angled_section="no" text_align="left" background_image_as_pattern="without_pattern"][vc_column width="2/3"][vc_column_text]The DPRA (OECD 442C) is an in chemico assay that models the first key event in the skin sensitization Adverse Outcome Pathway (AOP) - skin, protein reactivity. Compounds implicated in causing Allergic Contact Dermatitis (ACD) are generally electrophilic in nature. This assay identifies dermal sensitizers based on their reactivity with synthetic peptides containing the nucleophilic amino acid residues lysine and cysteine. Using LC/UV, conjugation of the test material with the peptides can be analyzed.

24 Aug Inflammatory Cytokine Expression

Posted at 16:48h in by Jane Ryan 0 Comments

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[vc_row css_animation="" row_type="row" use_row_as_full_screen_section="no" type="full_width" angled_section="no" text_align="left" background_image_as_pattern="without_pattern"][vc_column width="2/3"][vc_column_text]Many manufacturers of personal care and cosmetic products expect (along with their customers) that their products will not irritate the skin. Unfortunately, many ingredients (e.g. surfactants, preservatives, emulsifiers and many other organic substances) have an inherent irritating...

24 Aug Dermal Irritation Time to Toxicity

Posted at 16:47h in by Jane Ryan 0 Comments

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[vc_row css_animation="" row_type="row" use_row_as_full_screen_section="no" type="full_width" angled_section="no" text_align="left" background_image_as_pattern="without_pattern"][vc_column width="2/3"][vc_column_text]Product development/product stewardship scientists and formulators typically find it desirable to compare or rank order the skin irritation potential of candidate formulations, or to monitor the impact of changes in formulations or ingredients on skin tolerance. Foremost,...

24 Aug Skin Irritation Test (SIT, OECD 439)

Posted at 16:46h in by Jane Ryan 0 Comments

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[vc_row css_animation="" row_type="row" use_row_as_full_screen_section="no" type="full_width" angled_section="no" text_align="left" background_image_as_pattern="without_pattern"][vc_column width="2/3"][vc_column_text]Skin Irritation Test (SIT) in a Reconstructed Human Epidermis (RhE) Model Skin Irritation in the regulatory hazard classification and labeling context is defined as the production of reversible damage to skin following a defined chemical exposure. The Skin...